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12:e0181081

Cells were sorted using a FACS LSRFortessa (BD Biosciences) to determine the percentage of annexin V- and/or 7-AAD-positive cells using a 488 nm laser for annexin V-FITC and annexin V-PE and a 675 nm laser for7-AAD

Moreover, microsomal metabolism studies demonstrated that Dihexa had very low phase I metabolism, with an average intrinsic clearance of 2.72 l/min/mg and a half-life of 509.4 minutes